rabbit anti human hpv16 e6 antibody (Bioss)
Structured Review

Rabbit Anti Human Hpv16 E6 Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti human hpv16 e6 antibody/product/Bioss
Average 94 stars, based on 4 article reviews
Images
1) Product Images from "MiR-187 overexpression inhibits cervical cancer progression by targeting HPV16 E6"
Article Title: MiR-187 overexpression inhibits cervical cancer progression by targeting HPV16 E6
Journal: Oncotarget
doi: 10.18632/oncotarget.17516
Figure Legend Snippet: (A) Image shows 4% agarose electrophoresis of GAPDH, miR-187, HPV18 E6/E7, HPV16 E6/E7, and U6 PCR products. (B) Melting curves of GAPDH, miR-187, HPV18 E6/E7, HPV16 E6/E7, and U6 PCR products. M: 20-bp DNA marker.
Techniques Used: Electrophoresis, Marker
Figure Legend Snippet: (A) Detection of miR-187 expression levels after mimics transfection. (B) Relative expression of HPV18 E6/E7 and HPV16 E6/E7 mRNA; HPV16 E6 mRNA was decreased (*P < 0.05). (C) Corresponding increased expression of p53 protein compared to NC and blank groups (*P < 0.05). (D) Detection of miR-187 expression levels after inhibitor transfection. (E) Relative expression of HPV18 E6/E7 and HPV16 E6/E7 mRNA; HPV16 E6 mRNA was increased (*P < 0.05). (F) Decreased p53 protein expression (*P < 0.05).
Techniques Used: Expressing, Transfection
Figure Legend Snippet: (A) HPV16 E6 mRNA levels detected in the presence of miR-187 mimics or miR-187 inhibitor (**P < 0.01, ***P <0.001). (B) Western blot detection of HPV16 E6 protein levels in CaSki cells in the presence of miR-187 mimics (***P <0.001). (C) Bioinformatics prediction of the miR-187 binding sites of the HPV16 E6 3′ UTR. The mutated HPV16 E6 3′ UTR is also shown. (D) Luciferase activity of CHO cells co-transfected with miR-187 expression vector or empty vector and HPV16 E6 3′ UTR reporter plasmid or its mutant form for 48 h. Data shown are the means ± SD of three replicates (**P < 0.01).
Techniques Used: Western Blot, Binding Assay, Luciferase, Activity Assay, Transfection, Expressing, Plasmid Preparation, Mutagenesis
Figure Legend Snippet: Primer sequences
Techniques Used: Sequencing